It was the first time Barcelona had suffered such a blow in its history.
The match is part of a series of semi-finals in which four teams will advance to the semi-finals.
On Wednesday, Paris St. Germain beat Atalanta 2-1.
Red Bull Leipzig also beat Atletico Madrid 2-1 on Thursday.
There is now one game left - which will be played on Saturday night - between Manchester City and Lyon.
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